|Recommended Loading||2-5 μl/Lane|
|Concentration Typical Bands||100 ng/5μl|
|Concentration Other Bands||40 ng/5μl|
|Advised Electrophoresis Condition||8 cm,5% Agarose Gel, 0.5×TBE, 5 V/cm, 1 h|
|Contents bp||60, 80, 100, 120, 140, 160,180, 200, 220,240, 260, 280, 300|
|Mix Concentration||128 ng/μl|
The 20bp DNA Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs. The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.