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Gel loading dye, three-colour (6x) is a pre-mixed loading buffer with three tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. Edta is included to chelate magnesium (up to 10 mm) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue, xylene cyanol and orange g are the standard tracking dye for electrophoresis.