|Description||50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF , 50% lycerol.|
|10x Reaction Buffer||Buffer containing 25mM MgCl2|
|Applications||Hot Start and real time PCR, Multiplex PCR,Amplification of complex genomic and cDNA|
Hot start Taq DNA Polymerase is designed for Real-Time PCR and Hot- start PCR. It is modified with a special inhibition of PCR at room temperature. This will prevent primer dimers and other artifacts.
One unit is defined as the amount of enzyme that will incorporate 10n mole of dNTP into acid-insoluble material in 30 minutes at 74oC.