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Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5'→3' direction. Pfu DNA polymerase possesses 3'→5' exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high-fidelity. The fragments of Pfu DNA polymerase generated are blunt-ended.
One unit of Pfu DNA Polymerase incorporates 10 nmol of dNTP into acid-insoluble material in 30 min at 74°C.